Serial Dilution Advantages And Disadvantages Serial Dilution TechniquesĬompound Precipitation Many serial dilution techniques start with a compound in DMSO diluted into buffer.Īs this first step is the highest concentration of compound, the change in buffer solution could cause material to precipitate out of solution. The process is repeated, resulting in each successive well at a concentration increment lower than the previous well.Īs this technique has been the only available approach to IC 50 curve generation, the inherent drawbacks for this method are not often considered. Starting with a high concentration of drug in DMSO or buffer, they create their initial stock and then dilute across a series of wells.Ī small amount of the high concentration well is transferred to the next well, containing buffer or solvent. Serial Dilution Advantages And Disadvantages Series Of Wells Traditional Method: Serial Dilution Traditionally, scientists have approached the generation of IC 50 data in similar manners. The use of the eliminates these errors, enabling discoveries that would have been missed by other methods.īy coupling the Echo Liquid Handler with and, IC 50 data is generated more accurately and more efficiently than traditional methods. The setup of IC 50 curves have traditionally been a liquid handling challenge that is prone to error. Serial Dilution Advantages And Disadvantages Series Of WellsĬontinue making transfers until 1:100 and 1:10000 dilutions have been made.ĭIRECT DILUTION Different volumes of the same stock solution are transferred directly to the assay plate The generation of IC 50 or EC 50 data is a key component of drug discovery processes.Serial Dilution Advantages And Disadvantages Serial Dilution Techniques.
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